Monitoring Prunus Necrotic Ringspot Virus Infection by Hybridization with a cRNA Probe after in vitro Micrografting
نویسندگان
چکیده
منابع مشابه
Expression of Prunus Necrotic Ringspot Virus Coat Protein in E. coli
Background and Amis: Serological assay is considered as one of the best choices for conducting large number of infection tests. Recombinant DNA technology has been used for expression of virus coat protein (CP) gene in prokaryotic bacterial cells such as Escherichia coli and the recombinant CP (rCP) is used as immunogen in antibody production. Heterologous CP protein expression and purification...
متن کاملWhole-Genome Characterization of Prunus necrotic ringspot virus Infecting Sweet Cherry in China
Prunus necrotic ringspot virus (PNRSV) causes yield loss in most cultivated stone fruits, including sweet cherry. Using a small RNA deep-sequencing approach combined with end-genome sequence cloning, we identified the complete genomes of all three PNRSV strands from PNRSV-infected sweet cherry trees and compared them with those of two previously reported isolates.
متن کاملComparative analysis of ELISA, nonradioactive molecular hybridization and PCR for the detection of prunus necrotic ringspot virus in herbaceous and Prunus hosts
Three methods were compared for the detection of prunus necrotic ringspot virus in herbaceous and woody plants: DAS-ELISA, nonisotopic dot-blot hybridization and reverse transcriptional polymerase chain reaction (RT-PCR). When purified virus preparations were used, the detection limit of the RT-PCR technique was 1·28 pg mL whereas nonisotopic molecular hybridization and DAS-ELISA allowed detect...
متن کاملVertical transmission of Prunus necrotic ringspot virus: hitch-hiking from gametes to seedling.
The aim of this work was to follow Prunus necrotic ringspot virus (PNRSV) infection in apricot reproductive tissues and transmission of the virus to the next generation. For this, an analysis of viral distribution in apricot reproductive organs was carried out at different developmental stages. PNRSV was detected in reproductive tissues during gametogenesis. The virus was always present in the ...
متن کاملAdaptive covariation between the coat and movement proteins of prunus necrotic ringspot virus.
The relative functional and/or structural importance of different amino acid sites in a protein can be assessed by evaluating the selective constraints to which they have been subjected during the course of evolution. Here we explore such constraints at the linear and three-dimensional levels for the movement protein (MP) and coat protein (CP) encoded by RNA 3 of prunus necrotic ringspot ilarvi...
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ژورنال
عنوان ژورنال: Journal of the American Society for Horticultural Science
سال: 1995
ISSN: 0003-1062,2327-9788
DOI: 10.21273/jashs.120.6.928